Biological materials

Mutant

GP1888 (Δ[[gene|alsT]]::tet), available in [SW|Jörg Stülke]'s lab

BKE18120 (Δ[[gene|alsT]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE18120 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTTACCTCCCGTTTTC, downstream forward: _UP4_TAATATAAATATAAAACAAA

BKK18120 (Δ[[gene|alsT]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK18120 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTTACCTCCCGTTTTC, downstream forward: _UP4_TAATATAAATATAAAACAAA

GP1888 (Δ[[gene|alsT]]::tet), available in [SW|Jörg Stülke]'s lab [pubmed|32743959]

BKE18120 (Δ[[gene|alsT]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE18120 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTTACCTCCCGTTTTC, downstream forward: _UP4_TAATATAAATATAAAACAAA

BKK18120 (Δ[[gene|alsT]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK18120 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTTACCTCCCGTTTTC, downstream forward: _UP4_TAATATAAATATAAAACAAA

References

12823818, 25755103, 18763711, 29326168, 28835263, 31997474

12823818, 25755103, 18763711, 29326168, 28835263, 31997474, 30659158

The protein

Structure

[PDB|6CSE] (sodium/alanine symporter from Methanococcus maripaludis S2, 36% identity) [pubmed|30659158]